Role of a Cdc42p Effector Pathway in Recruitment of the Yeast Septins to the Presumptive Bud Site

• DOI: 10.1091/mbc.e05-08-0793
• Publication Date: 2005-12-22T01:39:08Z
• Authors: Masayuki Iwase; Jianying Luo; Satish Nagaraj; Mark Longtine; Hyong Bai Kim; Brian K. Haarer; Carlo Caruso; Zongtian Tong; John R. Pringle; Erfei Bi
• Keywords: Septin; Cell Cortex

The septins are GTP-binding, filament-forming proteins that involved in cytokinesis and other processes. In the yeast Saccharomyces cerevisiae, recruited to presumptive bud site at cell cortex, where they form a ring through which emerges. We report here wild-type cells, typically become detectable vicinity of several minutes before formation, but itself is first distinct structure forms. Septin recruitment depends on activated Cdc42p not normal pathway for bud-site selection. Recruitment occurs absence F-actin, formation delayed. Mutant phenotypes suppression data suggest effectors Gic1p Gic2p, previously implicated polarization actin cytoskeleton, also function septin recruitment. Two-hybrid, vitro protein binding, coimmunoprecipitation indicate this role involves direct interaction Gic with Cdc12p.