A Protein Interaction Map of the Mitotic Spindle

• DOI: 10.1091/mbc.e07-06-0536
• Publication Date: 2007-07-18T20:54:34Z
• Authors: Jonathan Wong; Yuko Nakajima; Stefan Westermann; Ching Shang; Jung-seog Kang; Crystal Goodner; Pantea Houshmand; Stanley Fields; Clarence S.M. Chan; David Drubin; Georjana Barnes; Tony Hazbun
• Keywords: Spindle pole body; Mitotic exit; Spindle checkpoint; Multipolar spindles; Aurora B kinase

The mitotic spindle consists of a complex network proteins that segregates chromosomes in eukaryotes. To strengthen our understanding the molecular composition, organization, and regulation spindle, we performed system-wide two-hybrid screen on 94 implicated function Saccharomyces cerevisiae. We report 604 predominantly novel interactions were detected multiple screens, involving 303 distinct prey proteins. uncovered pattern extensive between reflecting intricate organization spindle. Furthermore, observed connections kinetochore complexes chromatin-modifying used phosphorylation site mutants NDC80/TID3 to gain insights into possible phospho-regulation mechanisms. also present analyses She1p, protein interacts with Dam1 kinetochore/spindle complex. wealth presented here highlights extent which functions are integrated each other cellular activities.