Different Ubiquitin Signals Act at the Golgi and Plasma Membrane to Direct GAP1 Trafficking

• DOI: 10.1091/mbc.e07-06-0627
• Publication Date: 2008-04-24T01:25:58Z
• Authors: April L. Risinger; Chris A. Kaiser
• Keywords: Ubiquitin-conjugating enzyme

The high capacity general amino acid permease, Gap1p, in Saccharomyces cerevisiae is distributed between the plasma membrane and internal compartments according to availability of acids. When levels are low, Gap1p localized where it imports available acids from medium. sufficient imported, at endocytosed newly synthesized delivered vacuole; both sorting steps require ubiquitination. Although has been suggested that identical trans-acting factors ubiquitin acceptor sites involved processes, we define unique requirements for each ubiquitin-mediated delivery vacuole upon addition. Our finding distinct employ factors, ubiquitination on types demonstrates a previously unrecognized level specificity protein sorting.