DRhoGEF2andDiaphanousRegulate Contractile Force during Segmental Groove Morphogenesis in theDrosophilaEmbryo

• DOI: 10.1091/mbc.e07-12-1230
• Publication Date: 2008-02-21T02:23:30Z
• Authors: Shai Mulinari; Mojgan Padash Bar...; Udo Häcker
• Keywords: Formins; Apical constriction; Cell Cortex; Adherens junction

Morphogenesis of the Drosophila embryo is associated with dynamic rearrangement actin cytoskeleton mediated by small GTPases Rho family. These act as molecular switches that are activated guanine nucleotide exchange factors. One these factors, DRhoGEF2, plays an important role in constriction filaments during pole cell formation, blastoderm cellularization, and invagination germ layers. Here, we show DRhoGEF2 equally morphogenesis segmental grooves, which become apparent tissue infoldings mid-embryogenesis. Examination DRhoGEF2-mutant embryos indicates a for control shape changes groove morphogenesis. Overexpression ectoderm recruits myosin II to cortex induces contraction. At regression, enriched cells posterior undergo apical constriction, indicating regression active process. We further Formin Diaphanous required formation strengthens junctions epidermis. Morphological analysis suggests Dia regulates way distinct from DRhoGEF2. propose acts through Rho1 regulate acto-myosin but not Diaphanous-mediated F-actin nucleation