The Dynamics of Mammalian P Body Transport, Assembly, and Disassembly In Vivo

• DOI: 10.1091/mbc.e08-05-0513
• Publication Date: 2008-07-24T00:45:57Z
• Authors: Adva Aizer; Yehuda Brody; Lian Wee Ler; Nahum Sonenberg; Robert H. Singer; Yaron Shav-Tal
• Keywords: Dynamics

Exported mRNAs are targeted for translation or can undergo degradation by several decay mechanisms. The 5'-->3' machinery localizes to cytoplasmic P bodies (PBs). We followed the dynamic properties of PBs in vivo and investigated mechanism which scan cytoplasm. Using proteins decapping machinery, we asked whether actively cytoplasm a diffusion-based is sufficient. Live-cell imaging showed that were anchored mainly microtubules. Quantitative single-particle tracking demonstrated most exhibited spatially confined motion dependent on microtubule motion, whereas stationary PB pairs identified at centrosome. Some translocated long-range movements mobility was compared with mitochondria, endoplasmic reticulum, peroxisomes, SMN bodies, stress granules, diffusion coefficients calculated. Disruption network caused significant reduction together an induction assembly. However, FRAP measurements flux assembled components not affected such treatments. analysis enzyme Dcp2 nondynamic core protein, Dcp1 continuously exchanged This study reveals transport, it demonstrates how assembly disassembly integrate presence intact cytoskeleton.