The Exomer Coat Complex Transports Fus1p to the Plasma Membrane via a Novel Plasma Membrane Sorting Signal in Yeast
Chitin Synthase
0303 health sciences
Saccharomyces cerevisiae Proteins
Recombinant Fusion Proteins
Cell Membrane
Molecular Sequence Data
Coated Vesicles
Membrane Proteins
Saccharomyces cerevisiae
Protein Sorting Signals
Membrane Fusion
Exocytosis
Transcription Factor AP-1
Protein Transport
03 medical and health sciences
Two-Hybrid System Techniques
Mutation
Amino Acid Sequence
Sequence Alignment
trans-Golgi Network
DOI:
10.1091/mbc.e09-04-0324
Publication Date:
2009-10-08T01:35:54Z
AUTHORS (3)
ABSTRACT
Sorting of transmembrane cargo proteins to different cellular compartments is mediated by sorting signals that are recognized by coat proteins involved in vesicle biogenesis. We have identified a sorting signal in the yeast cell fusion protein Fus1p that is required for its transport from the trans-Golgi compartment to the plasma membrane. Transport of Fus1p from the trans-Golgi to the cell surface is dependent on Chs5p, a component of the multisubunit exomer complex. We show that Fus1p transport is also dependent on the exomer components Bch1p and Bud7p. Disruption of the clathrin adaptor protein complex 1 (AP-1) restores Fus1p localization to the cell surface in the absence of exomer, possibly by promoting an alternate, exomer-independent route of transport. Mutation of an IXTPK sequence in the cytosolic tail of Fus1p abolishes its physical interaction with Chs5p, results in mislocalization of Fus1p, and therefore causes a cell fusion defect. These defects are suppressed by disruption of AP-1. We suggest that IXTPK comprises a novel sorting signal that is recognized and bound by exomer leading to the capture of Fus1p into coated vesicles en route to the cell surface.
SUPPLEMENTAL MATERIAL
Coming soon ....
REFERENCES (33)
CITATIONS (52)
EXTERNAL LINKS
PlumX Metrics
RECOMMENDATIONS
FAIR ASSESSMENT
Coming soon ....
JUPYTER LAB
Coming soon ....