Mammalian pre-mRNA 3′ End Processing Factor CF Im68 Functions in mRNA Export

• DOI: 10.1091/mbc.e09-05-0389
• Publication Date: 2009-10-29T00:59:39Z
• Authors: Marc-David Ruepp; Chiara Aringhieri; Silvia Vivarelli; Stefano Cardinale; Simona Paro; Daniel Schümperli; Silvia M.L. Barabino
• Keywords: Cell Nucleus; Nucleocytoplasmic Transport Proteins; 0303 health sciences; Active Transport, Cell Nucleus; RNA-Binding Proteins; Receptors, Cytoplasmic and Nuclear; Karyopherins; Models, Biological; RNA Transport; Mice; Protein Subunits; 03 medical and health sciences; Ribonucleoproteins; RNA processing, SR proteins, RNA export;; Centrifugation, Density Gradient; NIH 3T3 Cells; RNA Precursors; Animals; Humans; Active Transport, Cell Nucleus; Animals; Cell Nucleus; Centrifugation, Density Gradient; HeLa Cells; Humans; Karyopherins; Mice; Models, Biological; NIH 3T3 Cells; Nucleocytoplasmic Transport Proteins; Protein Binding; Protein Subunits; RNA Precursors; RNA-Binding Proteins; Receptors, Cytoplasmic and Nuclear; Ribonucleoproteins; Ribosomes; Transcription, Genetic; mRNA Cleavage and Polyadenylation Factors; RNA 3' End Processing; RNA Transport; RNA 3' End Processing; Ribosomes; HeLa Cells; Protein Binding

Export of mRNA from the nucleus is linked to proper processing and packaging into ribonucleoprotein complexes. Although several observations indicate a coupling between 3' end formation export, it not known how these two processes are mechanistically connected. Here, we show that subunit mammalian pre-mRNA complex, CF I(m)68, stimulates export. I(m)68 shuttles cytoplasm in transcription-dependent manner interacts with export receptor NXF1/TAP. Consistent idea may act as novel adaptor for NXF1/TAP, promotes reporter well endogenous mRNAs, whereas silencing by RNAi results accumulation mRNAs nucleus. Moreover, associates 80S ribosomes but polysomes, suggesting part mRNP remodeled during initial stages translation. These reveal function factor