Nuclear Organization and Dynamics of 7SK RNA in Regulating Gene Expression

• DOI: 10.1091/mbc.e10-02-0105
• Publication Date: 2010-09-30T02:48:20Z
• Authors: Kannanganattu V. ...; Matthew Camiolo; Grace Chan; Vidisha Tripathi; Laurence Denis; Tetsuya Nakamura; Michael R. Hübner; David L. Spector
• Keywords: RNA polymerase II; Small nuclear RNA; P-TEFb; Transcription

Noncoding RNAs play important roles in various aspects of gene regulation. We have identified 7SK RNA to be enriched nuclear speckles or interchromatin granule clusters (IGCs), a subnuclear domain pre-mRNA processing factors. RNA, association with HEXIM 1 and 2, is involved the inhibition transcriptional elongation by polymerase II. Inhibition occurs via sequestration active P-TEFb kinase complex (CDK 9 Cyclin T1/T2a/b K) that phosphorylating C-terminal Our results demonstrate knock-down specific antisense oligonucleotides, mislocalization speckle constituents transcription-dependent manner, up-regulation II transcribed reporter locus. Furthermore, transiently associates stably integrated locus upon down-regulation its presence correlates efficient displacement from suggest plays role modulating available level sequestering speckles.