Regulation of cyclin-dependent kinase 4 translation through CUG-binding protein 1 and microRNA-222 by polyamines

• DOI: 10.1091/mbc.e11-01-0069
• Publication Date: 2011-07-08T15:04:11Z
• Authors: Lan Xiao; Yu-Hong Cui; Jaladanki N. Rao; Tongtong Zou; Lan Liu; Alexis Smith; Douglas J. Turner; Myriam Gorospe; Jian-Ying Wang
• Keywords: Colocalization

The amino acid-derived polyamines are organic cations that essential for growth in all mammalian cells, but their exact roles at the molecular level remain largely unknown. Here we provide evidence promote translation of cyclin-dependent kinase 4 (CDK4) by action CUG-binding protein 1 (CUGBP1) and microRNA-222 (miR-222) intestinal epithelial cells. Both CUGBP1 miR-222 were found to bind CDK4 mRNA coding region 3'-untranslated repressed synergistically. Depletion cellular increased cytoplasmic abundance levels, induced associations with mRNA, inhibited translation, whereas increasing levels decreased interaction miR-222, turn inducing expression. Polyamine-deficient cells exhibited an colocalization tagged processing bodies; this was abolished silencing miR-222. Together, our findings indicate polyamine-regulated modulate least part altering recruitment bodies.