Midbody assembly and its regulation during cytokinesis

• DOI: 10.1091/mbc.e11-08-0721
• Publication Date: 2012-01-26T01:30:41Z
• Authors: Chi-Kuo Hu; Margaret Coughlin; Timothy J. Mitchison
• Keywords: Midbody; Abscission

The midbody is a transient structure that connects two daughter cells at the end of cytokinesis, with principal function being to localize site abscission, which physically separates cells. Despite its importance, understanding assembly and regulation still limited. Here we describe how structural composition changes during progression throughout cytokinesis explore functional implications these changes. Deriving from midzones, midbodies are organized by set microtubule interacting proteins colocalize zone overlap in center. We found split into three subgroups relocalize different parts midbody: bulge, dark zone, flanking zone. characterized relocalizations defined domain requirements for key proteins: MKLP1, KIF4, PRC1. Two cortical proteins-anillin RhoA-localized presumptive abscission sites mature midbodies, where they may regulate endosomal sorting complex required transport machinery. Finally, role Plk1, regulator assembly. Our findings represent most detailed description maturation date help elucidate positioned regulated.