Dynactin's pointed-end complex is a cargo-targeting module

• DOI: 10.1091/mbc.e12-07-0496
• Publication Date: 2012-08-24T02:29:35Z
• Authors: Ting-Yu Yeh; Nicholas J. Quintyne; Brett R. Scipioni; D. Mark Eckley; Trina A. Schroer
• Keywords: Dynactin; Processivity

Dynactin is an essential part of the cytoplasmic dynein motor that enhances processivity and serves as adaptor allows to bind cargoes. Much known about dynactin's interaction with microtubules, but how it associates its diverse complement subcellular binding partners remains mysterious. It has been suggested cargo specification involves a group subunits referred "pointed-end complex." We used chemical cross-linking, RNA interference, protein overexpression characterize interactions within pointed-end complex explore contributes endomembranes. The Arp11 subunit, which caps one end Arp1 filament, p62, binds Arp1, are necessary for dynactin stability. These also allow nuclear envelope prior mitosis. p27 p25, by contrast, peripheral components can be removed without any obvious impact on integrity. lacking these shows reduced membrane binding. Depletion p25 results in impaired early recycling endosome movement, late movement unaffected, mitotic spindles appear normal. conclude bipartite structural domain stabilizes supports different structures.