The nanoscale spatial organization of B-cell receptors on immunoglobulin M– and G–expressing human B-cells

• DOI: 10.1091/mbc.e16-06-0452
• Publication Date: 2016-12-14T22:20:27Z
• Authors: Jinmin Lee; Prabuddha Sengupta; Joseph Brzostowski; Jennifer Lippinco...; Susan K. Pierce
• Keywords: breakpoint cluster region; Naive B cell; Surface Immunoglobulin

B-cell activation is initiated by the binding of antigen to receptor (BCR). Here we used dSTORM superresolution imaging characterize nanoscale spatial organization immunoglobulin M (IgM) and IgG BCRs on surfaces resting antigen--activated human peripheral blood B-cells. We provide insights into both fundamental process antigen-driven BCR clustering differences in IgM that may contribute characteristic responses naive memory B-cells antigen. evidence although reside highly heterogeneous protein islands vary size number single-molecule localizations, activated intrinsically maintain a high -frequency single isolated which likely represent monomers. are more clustered than cells form larger after activation. Small, dense clusters formed via protein-protein interactions present surface cells, induces these come together less dense, islands, governed, at least part, protein-lipid interactions.