Dual-color metal-induced and Förster resonance energy transfer for cell nanoscopy

• DOI: 10.1091/mbc.e17-05-0314
• Publication Date: 2018-02-14T12:40:19Z
• Authors: Anna M. Chizhik; Carina Wollnik; Daja Ruhlandt; Narain Karedla; Alexey I. Chizhik; Lara Hauke; Dirk Hähnel; Ingo Gregor; Jörg Enderlein; Florian Rehfeldt
• Keywords: Vinculin

We report a novel method, dual-color axial nanometric localization by metal-­induced energy transfer, and combine it with Förster resonance transfer (FRET) for resolving structural details in cells on the molecular level. demonstrate capability of this method cytoskeletal elements adhesions human mesenchymal stem cells. Our approach is based fluorescence-lifetime-imaging microscopy allows precise determination three-dimensional architecture stress fibers anchoring at focal adhesions, thus yielding crucial information to understand cell–matrix mechanics. In addition along z-axis, we use FRET gain distance between actin vinculin adhesions.