Dynein pulling forces counteract lamin-mediated nuclear stability during nuclear envelope repair

• DOI: 10.1091/mbc.e17-06-0374
• Publication Date: 2018-01-31T17:30:16Z
• Authors: Lauren Penfield; Brian Wysolmerski; Michael Mauro; Reza Farhadifar; Michael A. Martinez; Ronald Biggs; Hai-Yin Wu; Curtis Broberg; Daniel Needleman; Shirin Bahmanyar
• Keywords: Nuclear lamina

Recent work done exclusively in tissue culture cells revealed that the nuclear envelope (NE) ruptures and repairs interphase. The duration of NE depends on lamins; however, underlying mechanisms relevance to vivo events are not known. Here, we use Caenorhabditis elegans zygote analyze lamin’s role rupture repair vivo. Transient subsequent collapse induced by weaknesses lamina caused expression an engineered hypomorphic C. lamin allele. Dynein-generated forces position nuclei enhance severity transient cause collapse. Reduction dynein allows weakened network restrict nucleo–cytoplasmic mixing support stable recovery. Surprisingly, high incidence does contribute embryonic lethality, which is instead correlated with stochastic chromosome scattering resulting from premature collapse, suggesting tolerates losses compartmentalization during early embryogenesis. In sum, demonstrate counteracts promote prevent catastrophic thus provide first mechanistic analysis organismal context.