The synaptotagmin C2B domain calcium-binding loops modulate the rate of fusion pore expansion

• DOI: 10.1091/mbc.e17-11-0623
• Publication Date: 2018-02-14T17:41:46Z
• Authors: Mounir Bendahmane; Kevin P. Bohannon; Mazdak M. Bradberry; Tejeshwar C. Rao; Michael W. Schmidtke; Prabhodh S. Abbineni; Nara L. Chon; Sherleen Tran; Hai Lin; Edwin R. Chapman; Jefferson D. Knight; Arun Anantharam
• Keywords: Synaptotagmin 1; Chimera (genetics); C2 domain

In chromaffin cells, the kinetics of fusion pore expansion vary depending on which synaptotagmin isoform (Syt-1 or Syt-7) drives release. Our recent studies have shown that pores granules harboring Syt-1 expand more rapidly than those Syt-7. Here we sought to define structural specificity action at by manipulating Ca