The I1 dynein-associated tether and tether head complex is a conserved regulator of ciliary motility

• DOI: 10.1091/mbc.e18-02-0142
• Publication Date: 2018-03-07T17:25:12Z
• Authors: Gang Fu; Qian Wang; Nhan Phan; Paulina Urbanska; Ewa Joachimiak; Jianfeng Lin; Dorota Wloga; Daniela Nicastro
• Keywords: Dynein ATPase; Dynactin; Axoneme; Motile cilium

Motile cilia are essential for propelling cells and moving fluids across tissues. The activity of axonemal dynein motors must be precisely coordinated to generate ciliary motility, but their regulatory mechanisms not well understood. tether head (T/TH) complex was hypothesized provide mechanical feedback during beating because it links the motor domains I1 doublet microtubule. Combining genetic biochemical approaches with cryoelectron tomography, we identified FAP44 FAP43 (plus algae-specific, FAP43-redundant FAP244) as T/TH components. WT-mutant comparisons revealed that heterodimeric is required positional stability domains, stable anchoring CK1 kinase, proper phosphorylation IC138-subunit. also interacts inner arm d radial spoke 3, another important motility regulator. a conserved regulator plays an role in signaling pathway critical normal motility.