Temporal regulation of morphogenetic events inSaccharomyces cerevisiae

• DOI: 10.1091/mbc.e18-03-0188
• Publication Date: 2018-06-21T14:26:27Z
• Authors: Helen Lai; Jian-Geng Chiou; Anastasia Zhurikhina; Trevin R. Zyla; Denis Tsygankov; Daniel J. Lew
• Keywords: Septin; Cell polarity; CDC42; Polarity (international relations)

Tip growth in fungi involves highly polarized secretion and modification of the cell wall at growing tip. The genetic requirements for initiating are perhaps best understood model budding yeast Saccharomyces cerevisiae. Once is committed to enter cycle by activation G1 cyclin/cyclin-dependent kinase (CDK) complexes, polarity regulator Cdc42 becomes concentrated presumptive bud site, actin cables oriented toward that septin filaments assemble into a ring around site. Several minutes later, emerges. Here, we investigated mechanisms regulate timing these events single-cell level. Septin recruitment was delayed relative establishment, our findings suggest CDK-dependent “priming” facilitates Cdc42. Bud emergence initiation secretion, delay reflects time needed weaken sufficiently bud. Rho1 Rom2 occurred emergence, response local cell-wall weakening. This report reveals regulatory underlying morphogenetic yeast.