Polymer models reveal how chromatin modification can modulate force at the kinetochore

• DOI: 10.1091/mbc.e22-02-0041
• Publication Date: 2022-09-07T16:11:15Z
• Authors: Josh Lawrimore; Solenn C. de Larm...; Diana Cook; Brandon Friedman; Ayush Doshi; Elaine Yeh; Kerry Bloom
• Keywords: 0301 basic medicine; 03 medical and health sciences; Polymers; Chromosome Segregation; Centromere; Humans; Articles; DNA; Kinetochores; Microtubules; Chromatin

A key feature of chromosome segregation is the ability to sense tension between sister kinetochores. DNA kinetochores must be packaged in a way that sustains propagation from one kinetochore its sister, approximately 1 micron away. molecular bottlebrush consisting primary axis populated with crowded array side chains provides means build over length scales considerably larger than stiffness individual elements, is, polymer. Evidence for organization chromatin comes genetic, cell biological, and polymer modeling budding yeast centromere. In this study, we have used dynamic simulations recapitulate experimental observations structure. Several aspects spatial distribution proteins their response perturbation lack mechanistic understanding. Changes physical parameters bottlebrush, stiffness, loops directly impact architecture inner kinetochore. This study reveals an active participant building proposes mechanism feedback