Spatiotemporal analysis of axonal autophagosome–lysosome dynamics reveals limited fusion events and slow maturation

• DOI: 10.1091/mbc.e22-03-0111
• Publication Date: 2022-08-31T16:19:11Z
• Authors: Sydney E. Cason; Saurabh S. Mogre; Erika L. F. Holzbaur; Elena F. Koslover
• Keywords: 570; 0303 health sciences; 1.1 Normal biological development and functioning; Neurosciences; Autophagosomes; 610; Articles; Biological Sciences; Medical and Health Sciences; Axonal Transport; Membrane Fusion; Axons; 03 medical and health sciences; Spatio-Temporal Analysis; Underpinning research; Autophagy; Lysosomes; Developmental Biology

Autophagosome-lysosome fusion is required to clear cellular waste and maintain neuronal homeostasis. Computational modeling and microscopy elucidate the dynamics of autophagosome-lysosome interaction in axons. Each autophagosome fuses with only a few lysosomes, and autophagosomal maturation is limited by slow kinetics of inner membrane breakdown.