CLASP2 facilitates dynamic actin filament organization along the microtubule lattice

• DOI: 10.1091/mbc.e22-05-0149
• Publication Date: 2023-01-04T16:50:44Z
• Authors: N. C. Rodgers; E. J. Lawrence; A. V. Sawant; N. Efimova; G. Gonzalez-Vasquez; T. T. Hickman; I. Kaverina; M. Zanic
• Keywords: 0301 basic medicine; 0303 health sciences; Microtubule-actin crosslinking; Microtubules; Actins; Actin Cytoskeleton; 03 medical and health sciences; Humans; Brief Reports; Microtubule-Associated Proteins; Actin; CLASPs; Cytoskeleton; Protein Binding

CLASP2α directly cross-links multiple actin filaments to the microtubule lattice. CLASP2α-mediated cross-linking facilitates dynamic organization of actin filaments templated by microtubules. Furthermore, CLASP depletion results in disorganized actin fibers and reduced coalignment of actin fibers with microtubules, suggesting that microtubule–CLASP–actin interaction may influence overall cytoskeletal organization in cells.