CLASP2 facilitates dynamic actin filament organization along the microtubule lattice

• DOI: 10.1091/mbc.e22-05-0149
• Publication Date: 2023-01-04T16:50:44Z
• Authors: N. C. Rodgers; E. J. Lawrence; A. V. Sawant; N. Efimova; G. Gonzalez-Vasquez; T. T. Hickman; I. Kaverina; M. Zanic
• Keywords: Actin remodeling; MDia1; Actin-binding protein

Coordination between the microtubule and actin networks is essential for cell motility, neuronal growth cone guidance, wound healing. Members of CLASP (cytoplasmic linker-associated protein) family proteins have been implicated in cytoskeletal cross-talk microtubules networks; however, molecular mechanisms underlying role coordination are unclear. Here, we investigate CLASP2α's cross-linking function with F-actin. Our results demonstrate that CLASP2α cross-links F-actin to lattice vitro. We find ability retained by L-TOG2-S, a minimal construct containing TOG2 domain serine-arginine-rich region CLASP2α. Furthermore, promotes accumulation multiple filaments along microtubule, supporting up 11 landing events on single region. also facilitates dynamic organization polymerizing templated network, forming bridges individual microtubules. Finally, depletion CLASPs vascular smooth muscle cells disorganized fibers reduced coalignment microtubules, suggesting contribute higher-order structures. Taken together, our indicate can directly cross-link this microtubule-CLASP-actin interaction may influence overall cells.