Kazrin F is involved in apoptosis and interacts with BAX and ARC

• DOI: 10.1093/abbs/gmp065
• Publication Date: 2009-07-29T01:02:58Z
• Authors: Qiong Wang; Min Liu; Xin Li; Lu Chen; Hua Tang
• Keywords: 0303 health sciences; Intracellular Signaling Peptides and Proteins; Membrane Proteins; Apoptosis; Nerve Tissue Proteins; Astrocytoma; Kidney; Cell Line; Cytoskeletal Proteins; 03 medical and health sciences; Humans; Carrier Proteins; Signal Transduction; bcl-2-Associated X Protein

Kazrin has recently been identified as a functional protein that is involved in cell-cell junctions and signal transduction. Here, we new isoform, F, which 518 aa length 97 unique at the N-terminus. Knockdown of F using siRNA caused cell apoptosis marked decrease viability measured by MTT TUNEL assays. Co-immunoprecipitation analysis revealed interacts with ARC (apoptosis repressor caspase recruitment domain) Bax (Bcl-2-associated X protein). Co-localization cytoplasm was determined immunofluorescence analysis. These results suggested might play an important role regulating cellular interacting Bax.