Therapeutic potential of siRNA-mediated combined knockdown of the IAP genes (<italic>Livin</italic>, <italic>XIAP</italic>, and <italic>Survivin</italic>) on human bladder cancer T24 cells

• DOI: 10.1093/abbs/gmp118
• Publication Date: 2010-01-25T16:03:13Z
• Authors: Deyong Yang; Xishuang Song; Jianing Zhang; Lin Ye; Shujing Wang; Xiangyu Che; Jianbo Wang; Zhiwei Zhang; Lina Wang; Wei Shi
• Keywords: 0301 basic medicine; Survivin; Down-Regulation; X-Linked Inhibitor of Apoptosis Protein; Genetic Therapy; Inhibitor of Apoptosis Proteins; Neoplasm Proteins; 3. Good health; 03 medical and health sciences; Treatment Outcome; Urinary Bladder Neoplasms; Cell Line, Tumor; Humans; Gene Silencing; RNA, Small Interfering; Microtubule-Associated Proteins; Adaptor Proteins, Signal Transducing

Livin, X-linked inhibitor of apoptosis (XIAP), and Survivin are three well-known inhibitors of apoptosis almost exclusively over-expressed in cancer cells and are considered potent targets for cancer treatment. In the present study, we found that Livin, XIAP, and Survivin were simultaneously expressed in bladder cancer cells. We speculated that Livin, XIAP, and Survivin might have synergistic effects on cell growth and apoptosis. Our results confirmed that combined knockdown of all these three genes can synergistically inhibit the proliferation and transformation ability of high-grade bladder cancer T24 cells and promote the cell apoptotic sensitivity to chemotherapy. Furthermore, combined knockdown of Livin, XIAP, and Survivin can markedly increase the abundance of active caspase-3, active caspase-7, active caspase-9, and cytosolic Smac. Our findings imply that combined silencing of Livin, XIAP, and Survivin may be a potent multitargeted gene therapy for bladder cancer.