miR-330-3p controls cell proliferation by targeting early growth response 2 in non-small-cell lung cancer

• DOI: 10.1093/abbs/gmv032
• Publication Date: 2015-05-03T00:51:14Z
• Authors: Xuzhi Liu; Hanbing Shi; Bo Liu; Jianing Li; Yaxin Liu; Baiquan Yu
• Keywords: 0301 basic medicine; MicroRNAs; 0303 health sciences; 03 medical and health sciences; Lung Neoplasms; Carcinoma, Non-Small-Cell Lung; Cell Line, Tumor; Humans; Early Growth Response Protein 2; Cell Proliferation; Up-Regulation; 3. Good health

Non-small-cell lung cancer (NSCLC) is one of the most common cancers, and microRNAs (miRNAs) have been reported to play essential roles in NSCLC. Recent studies indicated that miR-330-3p expression up-regulated NSCLC samples tissues brain metastasis. In this study, up-regulation was confirmed 20 patient samples. Furthermore, over-expressed cell lines A549 H23, promotive function investigated regulating proliferation cycle distribution. To identify potential target genes NSCLC, miRNA prediction databases were used. Luciferase activity assay real-time RT-PCR analysis negatively correlated with early growth response 2 (EGR2). Moreover, it also found EGR2 mRNA contains two binding sites for miR-330-3p. Knock-down siRNA demonstrated a similar effect as over-expression lines. Taken together, our results show