Radiation history of Asian Asarum (sect. Heterotropa, Aristolochiaceae) resolved using a phylogenomic approach based on double-digested RAD-seq data

• DOI: 10.1093/aob/mcaa072
• Publication Date: 2020-04-11T11:10:39Z
• Authors: Yudai Okuyama; Nana Goto; Atsushi J Nagano; Masaki Yasugi; Goro Kokubugata; Hiroshi Kudoh; Zhechen Qi; Takuro Ito; Satoshi Kakishima; Takashi Sugawara
• Keywords: Aristolochiaceae; Sect; Phylogenomics

The genus Asarum sect. Heterotropa (Aristolochiaceae) probably experienced rapid diversification into 62 species centred on the Japanese Archipelago and Taiwan, providing an ideal model for studying island adaptive radiation. However, resolving phylogeny of this plant group using Sanger sequencing-based approaches has been challenging. To uncover radiation history Heterotropa, we employed a phylogenomic approach double-digested RAD-seq (ddRAD-seq) to yield sufficient number phylogenetic signals compared its utility with that approach.We first performance analysis based plastid matK trnL-F regions nuclear ribosomal internal transcribed spacer (nrITS), ddRAD-seq reduced set materials (83 accessions consisting 50 species, one subspecies six varieties). We also conducted more thorough analyses including reconstruction biogeographic comprehensive samples 135 54 subspecies, nine varieties outgroup species.Phylogenomic were superior resulted in fully resolved tree strong support 72.0-84.8 % (depending methods) branches. clarified found A. forbesii, only deciduous native mainland China, is sister evergreen (core Heterotropa) mostly distributed across Taiwan.The core was divided subclades, each which had narrow geographic distribution. Moreover, most estimated dispersal events (22 out 24) between adjacent areas, indicating range expansion geographically restricted throughout history. findings enhance our understanding remarkable lineages Taiwan.