Exosomal ncRNAs profiling of mycobacterial infection identified miRNA-185-5p as a novel biomarker for tuberculosis

• DOI: 10.1093/bib/bbab210
• Publication Date: 2021-05-13T11:09:59Z
• Authors: Aman Chandra Kaushik; Qiqi Wu; Li Lin; Haibo Li; Longqi Zhao; Zilu Wen; Yanzheng Song; Qihang Wu; Jin Wang; Xiaokui Guo; Hualin Wang; Xiaoli Yu; Dongqing Wei; Shulin Zhang
• Keywords: Biomarker Discovery

Abstract Background: There are ever increasing researches implying that noncoded RNAs (ncRNAs) specifically circular (circRNAs) and microRNAs (miRNAs) in exosomes play vital roles respiratory disease. However, the detailed mechanisms persist to be unclear mycobacterial infection. Methods: In order detect circRNAs miRNAs expression pattern potential biological function tuberculosis, we performed immense parallel sequencing for exosomal ncRNAs from THP-1-derived macrophages infected by Mycobacterium tuberculosis H37Ra, bovis BCG control Streptococcus pneumonia, respectively uninfected normal cells. Besides, were used verify validation of differential miRNAs, monocytes PBMCs clinical plasma samples further validate differentially expressed miR-185-5p. Results: Many associated with infection recognized. Extensive enrichment analyses illustrate major effects altered expression. Moreover, miRNA–mRNA circRNA–miRNA networks created expected reveal their interrelationship. Further, significant based on Exo-BCG, Exo-Ra Exo-Control, evaluated, target mRNAs analyzed. Eventually, miR-185-5p was collected as a promising biomarker tuberculosis. Conclusion: Our findings provide new vision exploring functions screening novel biomarkers. To sum up, might represent useful functional biomarkers pathogenesis diagnosis.