MOSGA: Modular Open-Source Genome Annotator

• DOI: 10.1093/bioinformatics/btaa1003
• Publication Date: 2020-11-18T14:35:16Z
• Authors: Roman Martin; Thomas Hackl; Georges Hattab; Matthias G Fischer; Dominik Heider
• Keywords: Genomics (q-bio.GN); 0301 basic medicine; 03 medical and health sciences; Genome; FOS: Biological sciences; Eukaryota; Quantitative Biology - Genomics; Software

Abstract

Motivation The generation of high-quality assemblies, even for large eukaryotic genomes, has become a routine task for many biologists thanks to recent advances in sequencing technologies. However, the annotation of these assemblies—a crucial step toward unlocking the biology of the organism of interest—has remained a complex challenge that often requires advanced bioinformatics expertise.

Results Here, we present MOSGA (Modular Open-Source Genome Annotator), a genome annotation framework for eukaryotic genomes with a user-friendly web-interface that generates and integrates annotations from various tools. The aggregated results can be analyzed with a fully integrated genome browser and are provided in a format ready for submission to NCBI. MOSGA is built on a portable, customizable and easily extendible Snakemake backend, and thus, can be tailored to a wide range of users and projects.

Availability and implementation We provide MOSGA as a web service at https://mosga.mathematik.uni-marburg.de and as a docker container at registry.gitlab.com/mosga/mosga: latest. Source code can be found at https://gitlab.com/mosga/mosga

Supplementary information Supplementary data are available at Bioinformatics online.