In silico identification of oncogenic potential of fyn-related kinase in hepatocellular carcinoma

• DOI: 10.1093/bioinformatics/bts715
• Publication Date: 2012-12-25T03:12:57Z
• Authors: Jia-Shing Chen; Wei-Shiang Hung; Hsiang-Han Chan; Shaw-Jenq Tsai; H. Sunny Sun
• Keywords: FYN; Identification

Abstract Motivation: Cancer development is a complex and heterogeneous process. It estimated that 5–10% of human genes probably contribute to oncogenesis, whereas current experimentally validated cancer only cover 1% the genome. Thus hundreds may still remain be identified. To search for new play roles in carcinogenesis facilitate research, we developed systematic workflow use information saved previously established tumor-associated gene (TAG) database. Results: By exploiting conserved protein domains from TAG, identified 183 potential TAGs. As proof-of-concept, one predicted oncogene, fyn-related kinase (FRK), which shows an aberrant digital expression pattern liver cells, was selected further investigation. Using 68 paired hepatocellular carcinoma samples, found FRK up-regulated 52% cases (P < 0.001). Tumorigenic assays performed Hep3B HepG2 cell lines revealed significant correlation between level invasiveness, suggesting positive regulator invasiveness cells. Conclusion: These findings implied multitalented signal transduction molecule produces diverse biological responses different types various microenvironments. In addition, our data demonstrated accuracy computational prediction suggested other TAGs can targets future research. Availability: The TAG database available online at Bioinformatics Center website: http://www.binfo.ncku.edu.tw/TAG/. Contact: [e-mail] Supplementary information: are online.