AgIn: measuring the landscape of CpG methylation of individual repetitive elements

• DOI: 10.1093/bioinformatics/btw360
• Publication Date: 2016-06-19T00:18:20Z
• Authors: Yuta Suzuki; Jonas Korlach; Stephen W. Turner; Tatsuya Tsukahara; Junko Taniguchi; Wei Qu; Kazuki Ichikawa; Jun Yoshimura; Hideaki Yurino; Yuji Takahashi; Jun Mitsui; Hiroyuki Ishiura; Shoji Tsuji; Hiroyuki Takeda; Shinichi Morishita
• Keywords: CpG site

Determining the methylation state of regions with high copy numbers is challenging for second-generation sequencing, because read length insufficient to map reads uniquely, especially when repetitive are long and nearly identical each other. Single-molecule real-time (SMRT) sequencing a promising method observing such regions, it not vulnerable GC bias, produces lengths, its kinetic information sensitive DNA modifications.