CRISPR-DAV: CRISPR NGS data analysis and visualization pipeline

• DOI: 10.1093/bioinformatics/btx518
• Publication Date: 2017-08-10T19:14:46Z
• Authors: Xuning Wang; Charles Tilford; Isaac Neuhaus; Gabe Mintier; Qi Guo; John N Feder; Stefan Kirov
• Keywords: Indel

The simplicity and precision of CRISPR/Cas9 system has brought in a new era gene editing. Screening for desired clones with CRISPR-mediated genomic edits large number samples is made possible by next generation sequencing (NGS) due to its multiplexing. Here we present CRISPR-DAV (CRISPR Data Analysis Visualization) pipeline analyze the CRISPR NGS data high throughput manner. In pipeline, Burrows-Wheeler Aligner Assembly Based ReAlignment are used small indel detection, results presented comprehensive set charts interactive alignment view.CRISPR-DAV available at GitHub Docker Hub repositories: https://github.com/pinetree1/crispr-dav.git https://hub.docker.com/r/pinetree1/crispr-dav/[e-mail].