PET-MRI nanoparticles imaging of blood–brain barrier damage and modulation after stroke reperfusion

• DOI: 10.1093/braincomms/fcaa193
• Publication Date: 2020-11-11T14:24:46Z
• Authors: Justine Debatisse; Omer Faruk Eker; Océane Wateau; Tae-Hee Cho; Marlène Wiart; David Ramonet; Nicolas Costes; Inés Mérida; Christelle Léon; Maya Dia; Mélanie Paillard; Joachim Confais; Fabien Rossetti; Jean-Baptiste Lan...; Thomas Troalen; Thibaut Iecker; Didier Le Bars; Sophie Lancelot; Baptiste Bouchier; Anne-Claire Lukas...; Adrien Oudotte; Norbert Nighoghos...; Michel Ovize; Hugues Contamin; François Lux; Olivier Tillement; Emmanuelle Canet-...
• Keywords: Stroke; Modulation (music); Brain damage

In an acute ischaemic stroke, understanding the dynamics of blood-brain barrier injury is particular importance for prevention symptomatic haemorrhagic transformation. However, available techniques assessing permeability are not quantitative and little used in context reperfusion therapy. Nanoparticles cross healthy or impaired through combined passive active processes. Imaging quantifying their transfer rate could better characterize damage refine delivery neuroprotective agents. We previously developed original endovascular stroke model treated by mechanical thrombectomy followed positron emission tomography-magnetic resonance imaging. Cerebral capillary was quantified two molecule sizes: small clinical gadolinium Gd-DOTA (<1 nm) AGuIX® nanoparticles (∼5 brain theranostics. On dynamic contrast-enhanced magnetic imaging, baseline constant Ktrans 0.94 [0.48, 1.72] 0.16 [0.08, 0.33] ×10-3 min-1, respectively, normal parenchyma, consistent with respective sizes, 1.90 [1.23, 3.95] 2.86 [1.39, 4.52] min-1 choroid plexus, confirming higher than parenchyma. At early reperfusion, both significantly within area compared to contralateral hemisphere; 2.23 [1.17, 4.13] 0.82 [0.46, 1.87] nanoparticles, respectively. With also increased growth areas, suggesting added value AGuIX®. Finally, lower lesion plexus a drug-treated group (ciclosporin A, n = 7) placebo (n 5). quantification can monitor treatment effect after reperfusion.