Characterization of neuroinflammatory positron emission tomography biomarkers in chronic traumatic encephalopathy

• DOI: 10.1093/braincomms/fcac019
• Publication Date: 2022-02-01T20:12:43Z
• Authors: Cassis Varlow; Ashley C. Knight; Paul McQuade; Neil Vasdev
• Keywords: Translocator protein; Chronic traumatic encephalopathy

Abstract Chronic traumatic encephalopathy is a neurological disorder associated with head trauma and confirmed upon autopsy. PET imaging of chronic may provide means to move towards ante-mortem diagnosis therapeutic intervention following brain injuries. Characterization the neuroinflammatory biomarkers, 18 kDa translocator protein monoamine oxidase-B was conducted using [3H]PBR-28 [3H]L-deprenyl, respectively, in post-mortem tissue. displayed high specific binding both (95.40 ± 1.87%; n = 11 cases) healthy controls (89.89 8.52%, 3 cases). Cell-type expression by immunofluorescence microglia, astrocyte macrophage markers. [3H]L-deprenyl also (96.95 1.43%; 12 (93.24 0.43%; 2 cases), distribution co-localized astrocytes immunofluorescence. Saturation analysis performed quantify target density control Using [3H]PBR-28, 177.91 56.96 nM (n 7 cases; mean standard deviation); however, highly variable (345.84 372.42 nM; deviation) measured encephalopathy. quantified 304.23 115.93 8 tissue similar tissues (365.80 128.55 deviation). A two-sample t-test determined no significant difference values between (P > 0.05), albeit trend increased targets observed To our knowledge, this work represents first vitro characterization reveals variability pathology These preliminary findings will be considered when designing studies after injury for ultimate goal vivo.