Reference range for serum neurofilament light chain: findings from healthy Thai adults

• DOI: 10.1093/braincomms/fcaf166
• Publication Date: 2025-04-29T08:32:33Z
• Authors: Nontapat Sukhonpa...; Tatchaporn Ongphi...; Ekdanai Uawithya; Jiraporn Jitprapa...; Natthapon Rattana...; Naraporn Prayoonw...; Sasitorn Siritho
• Keywords: Reference range; Chain (unit)

Abstract Serum neurofilament light chain is a notable biomarker for detecting axonal injury and has shown significant potential clinical applications. Establishing reference interval cut-off level critical step towards implementing serum in routine practice. In this study, we aimed to establish range of chains the Thai population. Blood samples were collected from healthy adults without history neurological diseases screened at Siriraj Hospital. The relationship between age, sex log10-transformed levels was analysed using linear regression. A crude calculated as 2.5–97.5th percentile values. An age-normative curve derived generalized additive model location, scale shape. total 223 subjects (96 males 127 females) aged 18–70 years recruited. Male (P = 0.008) older age < 0.001) significantly associated with higher levels. median observed values 5.8 pg/ml (95% confidence 5.4–6.2), ranging 1.0 18.4 pg/ml, 2.3–15.9 pg/ml. intervals by group follows: 20–29 (n 57): 1.7–8.7 pg/ml; 30–39 58): 2.5–10.6 40–49 59): 3.5–14.3 50–59 37): 4.7–15.8 60–69 12): 4.2–18.2 predicted 97.5th 8.2, 9.9, 11.7, 14.6 19.9 ages 20, 30, 40, 50 60, respectively. This study first Thailand. upper closely aligned those previously reported other studies, providing robust framework implementation. However, further validation larger cohorts among individuals warranted.