HPLC Method for Naproxen Determination in Human Plasma and Its Application to a Pharmacokinetic Study in Turkey

• DOI: 10.1093/chromsci/bmt080
• Publication Date: 2013-06-22T00:23:48Z
• Authors: B. Yilmaz; A. Asci; A. F. Erdem
• Keywords: Trifluoroacetic acid; Phosphate buffered saline

A simple high-performance liquid chromatography method has been developed for the determination of naproxen in human plasma. The was validated on an Ace C18 column using ultraviolet detection. mobile phase consisted 20 mM phosphate buffer (pH 7) containing 0.1% trifluoroacetic acid–acetonitrile (65:35, v/v). calibration curve linear between concentration ranges 0.10 and 5.0 µg/mL. Intra-day inter-day precision values plasma were less than 4.84, accuracy (relative error) better 3.67%. extraction recovery from 91.0 98.9%. limits detection quantification 0.03 µg/mL, respectively. Also, this assay applied to determine pharmacokinetic parameters six healthy Turkish volunteers who had given 220 mg naproxen.