Functional vascular smooth muscle cells derived from human induced pluripotent stem cells via mesenchymal stem cell intermediates

• DOI: 10.1093/cvr/cvs253
• Publication Date: 2012-09-01T20:38:33Z
• Authors: Vivek K. Bajpai; Panagiotis Mistri...; Yuin-Han Loh; George Q. Daley; Stelios T. Andreadis
• Keywords: Calponin; Regenerative Medicine; Directed differentiation

Smooth muscle cells (SMC) play an important role in vascular homeostasis and disease. Although adult mesenchymal stem (MSC) have been used as a source of contractile SMC, they suffer from limited proliferation potential culture senescence, particularly when originating older donors. By comparison, human induced pluripotent (hiPSC) can provide unlimited functional SMC for autologous cell-based therapies creating models Our goal was to develop efficient strategy derive functional, hiPSC. We developed robust, stage-wise, feeder-free hiPSC differentiation into through intermediate stage multipotent MSC, which could be coaxed differentiate fat, bone, cartilage, muscle. At this stage, the were highly proliferative displayed higher clonogenic reduced senescence compared with parental hair follicle cells. In addition, exposed medium, myogenic proteins such α-smooth actin, calponin, myosin heavy chain significantly upregulated robust fibrillar organization, suggesting development phenotype. Indeed, tissue constructs prepared these exhibited high levels contractility response receptor- non-receptor-mediated agonists. stage-wise that enabled population MSC. The yield MSC derivation suggests our may facilitate acquisition large numbers required regenerative medicine or studying disease pathophysiology.