Curation of a reference database of COI sequences for insect identification through DNA metabarcoding: COins

• DOI: 10.1093/database/baac055
• Publication Date: 2022-07-07T12:07:56Z
• Authors: Giulia Magoga; Giobbe Forni; Matteo Brunetti; Aycan Meral; Alberto Spada; Alessio De Biase; Matteo Montagna
• Keywords: Barcode; Identification; DNA Barcoding; Reference database

DNA metabarcoding is a widespread approach for the molecular identification of organisms. While associated wet-lab and data processing procedures are well established highly efficient, reference databases taxonomic assignment can be implemented to improve accuracy identifications. Insects among organisms which DNA-based most commonly used; yet, DNA-metabarcoding database specifically curated their species using software requiring local lacking. Here, we present COins, 5' region cytochrome c oxidase subunit I sequences (COI-5P) insects that includes over 532 000 representative >106 formatted QIIME2 platform. Through combination automated manually steps, developed this starting from all COI available in Barcode Life Data System insects, focusing on comply with several standards, including species-level identification. COins was validated previously published (bulk samples Malaise traps) its efficiency compared other publicly (not specific insects). allow an increase up 30% identifications thus represent valuable resource insects' data, especially when needed https://doi.org/10.6084/m9.figshare.19130465.v1.