LeishDB: a database of coding gene annotation and non-coding RNAs in Leishmania braziliensis

• DOI: 10.1093/database/bax047
• Publication Date: 2017-05-19T07:08:39Z
• Authors: Felipe Torres; Raúl Arias-Carrasco; José C. Caris-Mal...; Aldina Barral; Vinicius Maracaja...; Artur T. L. De Qu...
• Keywords: ORFS; Leishmania braziliensis; Gene Annotation

Leishmania braziliensis is the etiological agent of cutaneous leishmaniasis, a disease with high public health importance, affecting 12 million people worldwide. Although its genome sequence was originally published in 2007, two reference annotations still presents at least 80% genes simply classified as hypothetical or putative proteins. Furthermore, it notable absence non-coding RNA (ncRNA) sequences from species databases. These poorly annotated coding and ncRNAs could be important players for understanding this protozoan biology, mechanisms behind host-parasite interactions control. Herein, we performed new prediction annotation L. protein-coding RNAs, using recently developed predictive algorithms updated In summary, identified 11 491 ORFs, 5263 (45.80%) them associated proteins available Moreover, first time repertoire 243 belonging to different classes distributed along genome. The accuracy our predictions verified by transcriptional evidence RNA-seq, confirming that they are actually generating real transcripts. data were organized repository named LeishDB (www.leishdb.com), which represents an improvement on publicly related genomic braziliensis. This information can useful future genomics, transcriptomics metabolomics studies; being additional tool pipelines novel studies complexity, organization, development innovative methodologies control diagnostics. Database URL:www.leishdb.com