Isolation and Expression Profiling of Genes Upregulated in the Peripheral Blood Cells of Systemic Lupus Erythematosus Patients

• DOI: 10.1093/dnares/dsi020
• Publication Date: 2007-02-22T20:07:50Z
• Authors: Taeko Ishii; Hiroaki Onda; Akie Tanigawa; Shiro Ohshima; Hiroshi Fujiwara; Toru Mima; Yoshinori Katada; Hitoshi Deguchi; Masaki Suemura; Tadao Miyake; Kunio Miyatake; Ichiro Kawase; Hanjun Zhao; Yoshiaki Tomiyama; Yukihiko Saeki; Hiroshi Nojima
• Keywords: Male; Purpura, Thrombocytopenic, Idiopathic; 0303 health sciences; Proteome; Gene Expression Profiling; Up-Regulation; 3. Good health; 03 medical and health sciences; Leukocytes, Mononuclear; Humans; Lupus Erythematosus, Systemic; Female; Gene Library; Oligonucleotide Array Sequence Analysis

We have identified the genes whose expressions are augmented in blood cells of patients with systemic lupus erythematosus (SLE) using 'stepwise subtraction' technique along microarray analysis. The expression levels these were assessed by quantitative real-time reverse transcription polymerase chain reaction (RT-PCR) 31 SLE and 30 healthy controls. found that following eight significantly increased patients; interferon (IFN)-alpha-inducible protein 27 (IFI27), IFN-alpha-inducible IFI-15K (G1P2), IFN stimulated gene 20 kDa (ISG20), epithelial stromal interaction 1 (EPSTI1), defensin-alpha (DEFA3), amphiregulin (AREG) two unknown function (BLAST accession nos AL050290 AY358224 = SLED1). In comparison idiopathic thrombocytopenic purpura (ITP), an organ-specific autoimmune disease, IFI27, G1P2 SLED1 preferentially upregulated SLE. contrast, AREG more highly expressed ITP than correlated changes clinical/laboratory features ISG20, EPSTI1 lymphocyte counts. Genes linked to well known influence SLE, but several other novel unrelated signaling we report here would be useful understand pathophysiology