Draft Genome of the Pearl Oyster Pinctada fucata: A Platform for Understanding Bivalve Biology

• DOI: 10.1093/dnares/dss005
• Publication Date: 2012-02-08T05:07:43Z
• Authors: T. Takeuchi; T. Kawashima; R. Koyanagi; F. Gyoja; M. Tanaka; T. Ikuta; E. Shoguchi; M. Fujiwara; C. Shinzato; K. Hisata; M. Fujie; T. Usami; K. Nagai; K. Maeyama; K. Okamoto; H. Aoki; T. Ishikawa; T. Masaoka; A. Fujiwara; K. Endo; H. Endo; H. Nagasawa; S. Kinoshita; S. Asakawa; S. Watabe; N. Satoh
• Keywords: Pinctada fucata

The study of the pearl oyster Pinctada fucata is key to increasing our understanding molecular mechanisms involved in biosynthesis and biology bivalve molluscs. We sequenced ∼1150-Mb genome at ∼40-fold coverage using Roche 454 GS-FLX Illumina GAIIx sequencers. sequences were assembled into contigs with N50 = 1.6 kb (total contig assembly reached 1024 Mb) scaffolds 14.5 kb. AT-rich, a GC content 34%. DNA transposons, retrotransposons, tandem repeat elements occupied 0.4, 1.5, 7.9% genome, respectively (a total 9.8%). Version 1.0 P. draft contains 23 257 complete gene models, 70% which are supported by corresponding expressed sequence tags. genes include those reported have an association bio-mineralization. Genes encoding transcription factors signal transduction molecules present numbers comparable genomes other metazoans. Genome-wide phylogeny suggests that lophotrochozoan represents distinct clade from ecdysozoans. Our thus provides platform for identification selection markers calcification, knowledge will be important industry.