Proteomics insights into the responses of Saccharomyces cerevisiae during mixed-culture alcoholic fermentation with Lachancea thermotolerans

• DOI: 10.1093/femsec/fiz126
• Publication Date: 2019-08-16T13:31:38Z
• Authors: Chuantao Peng; Birgit Andersen; Samina Arshid; Martin R Larsen; Helena Albergaria; Rene Lametsch; Nils Arneborg
• Keywords: Ethanol Fermentation

ABSTRACT The response of Saccharomyces cerevisiae to cocultivation with Lachancea thermotolerans during alcoholic fermentations has been investigated using tandem mass tag (TMT)-based proteomics. At two key time-points, S. was sorted from single and mixed flow cytometry sorting. Results showed that the purity above 96% throughout whole mixed-culture fermentation, thereby validating our sorting methodology. By comparing protein expression without L. thermotolerans, 26 proteins were identified as significantly regulated at early death phase (T1), 32 late (T2) in cultures. T1, involved endocytosis, increasing nutrient availability, cell rescue resistance stresses upregulated, proline synthesis apoptosis downregulated. T2, stress responses up- downregulated, respectively. These data indicate stressed by presence both defensive fighting strategies keep itself a dominant position, it T2 relieved stress, perhaps its enzymatic machinery ensure better survival.