Whole genome sequence of an edible mushroom Strobilomyces alpinus (Boletaceae)

• DOI: 10.1093/g3journal/jkaf080
• Publication Date: 2025-04-10T03:09:36Z
• Authors: Li-Hong Han; Yan-Ting Duan; Han-Yan Yuan; Yan-Jia Hao; Chao Liu
• Keywords: Comparative Genomics; Sequence assembly; Genome size; Gene cluster

Abstract Strobilomyces alpinus is a unique and significant mushroom endemic to southwestern central China, characterized by its exclusive subalpine distribution strong host preference for Abies spp. The biological genetic studies of this are scarce, which significantly hinders research on molecular breeding evolutionary patterns. In study, we report the de novo sequencing assembly S. genome using DNBSEQ-T7 third-generation Pacific Biosciences platform. total size was approximately 58.75 Mb, with GC content 54.87%. produced 68 contigs, an N50 length 4.03 Mb. comprises 11,761 annotated protein-coding genes, including 813 CAZyme-coding 182 Cytochrome P450 1,821 candidate pathogenicity-related genes. non-coding RNA prediction results indicated presence 532 rRNAs, 62 small RNAs, 98 tRNAs in genome. Notably, there high degree repetition (44.28%) within Additionally, identified 16 secondary metabolite gene clusters, seven NRPS-like five terpene one fungal-RiPP-like cluster, RiPP-like T1PKS T1PKS-NRPS linkage cluster. Several important metabolic pathways, terpenoid backbone biosynthesis, porphyrin metabolism, folate have been elucidated. whole-genome sequence can serve as reference investigations bioactive compounds medicinal value commercial production.