Organellar Genomes of White Spruce (Picea glauca): Assembly and Annotation

• DOI: 10.1093/gbe/evv244
• Publication Date: 2015-12-09T13:36:30Z
• Authors: Shaun D. Jackman; René L. Warren; Ewan A. Gibb; Benjamin P. Vande...; Hamid Mohamadi; Justin Chu; Anthony Raymond; Stephen Pleasance; Robin Coope; Mark R. Wildung; Carol E. Ritland; Jean Bousquet; Steven J. M. Jones; Joerg Bohlmann; Inanç Birol
• Keywords: Sequence assembly; Nuclear gene

The genome sequences of the plastid and mitochondrion white spruce ( Picea glauca ) were assembled from whole-genome shotgun sequencing data using ABySS. contained reads both nuclear organellar genomes, genomes abundant in as each cell harbors hundreds mitochondria plastids. Hence, assembly 123-kb 5.9-Mb mitochondrial accomplished by analyzing sets primarily representing low coverage genome. annotated for their coding genes, ribosomal RNA, transfer RNA. Transcript abundances genes quantified three developmental tissues five mature RNA-seq experiments. C-to-U RNA editing was observed majority four events noted to modify ACG codons create cryptic AUG start codons. informatics methodology presented this study should prove useful assemble other plant species data.