The male sterility-associated pcf gene and the normal atp9-1 gene in Petunia are located on different mitochondrial DNA molecules.

• DOI: 10.1093/genetics/129.3.885
• Publication Date: 2021-01-13T18:04:56Z
• Authors: O Folkerts; M R Hanson
• Keywords: Petunia; Cytoplasmic male sterility

A mitochondrial DNA (mtDNA) region termed the S-pcf locus has previously been correlated with cytoplasmic male sterility (CMS) in Petunia. In order to understand relationship of homologous sequences found elsewhere mtDNAs both CMS and fertile lines, structure genome Petunia line 3688 was determined by cosmid walking. The locus, which includes only copies genes for NADH dehydrogenase subunit 3 (nad3) small ribosomal protein 12 (rps12) be located on a circular map 396 kb, while second almost identical 407 kb carries 18S 5S rRNA (rrn18 rrn5), copy conserved unidentified gene (orf25), known functional atp9. Three different recombination repeat were six genomic environments, predicting sub-genomic circles 277, 266 130 kb. ratio atp9 mtDNA approximately 1.5 1, indicating that molecules carrying these differ abundance. Comparison organization master circle 3704 reveals numerous changes orientation ten sectors.