LIPID REGULATION BY DGA1, CUT6, AND SRE1 CONTRIBUTE TO THE GENOTOXIC STRESS RESPONSE IN FISSION YEAST

• DOI: 10.1093/geroni/igac059.1610
• Publication Date: 2022-12-20T18:20:11Z
• Authors: Wilber Escorcia; Mauricio Dominguez; Maryam Khzyr; Sam Burek; Jack Pluth; Meghan Graber; Hayley Barta; Alex Rausenberger
• Keywords: Lipid droplet; Cell Physiology

Abstract Lipids are essential for cell growth and maintenance, intracellular signaling, cellular energetics. When exposed to environmental insults, cells activate lipid metabolism programs that facilitate the response, recovery, exit from stressful conditions. Failure regulate storage utilization is associated with accelerated aging, which compounds genomic instability. In this study, we test physiological consequences of disruptions three regulator genes frequently found be mutated in human cancers. Since products these show functional homology fission yeast, examine how deregulation by two enzymes (Dga1DGAT2 Cut6ACC) one transcription factor (Sre1SREBF1) influence response genotoxic stress organism. Using microscopy quantification staining, observe abnormal homeostatic control levels lacking Dga1, Cut6, Sre1. These phenotypes enhanced sub-lethal doses DNA damage (UV-C light) altered fitness proliferation. Furthermore, genotoxicity, mutants exhibit disrupted nuclear segregation, dimensions, lifespan. data suggest mechanisms may contribute deregulated metabolic environments prematurely aged tumors