Leucine-rich repeat kinase 2 associates with lipid rafts

• DOI: 10.1093/hmg/ddm013
• Publication Date: 2007-03-07T03:00:52Z
• Authors: Taku Hatano; Shin-ichiro Kubo; Satoshi Imai; Masahiro Maeda; Kiyoshi Ishikawa; Yoshikuni Mizuno; Nobutaka Hattori
• Keywords: LRRK2; Lipid raft; Leucine-rich repeat

Leucine-Rich Repeat Kinase 2 (LRRK2) is a causative gene for the autosomal dominant form of Parkinson's disease (PD). The encodes approximately 280 kDa LRRK2 protein composed domains such as leucine-rich repeats, Ras in complex proteins (Roc) followed by C-terminal Roc (COR), mitogen-activated kinase (MAPKKK) and WD40. However, normal function well its contribution to pathogenesis PD remains largely unknown. Here we describe localization Golgi apparatus, plasma membrane synaptic vesicles cultured cells including mouse primary neurons. association resists solubilization ice-cold 1% Triton X-100, indicating through lipid rafts. To investigate whether mutations found patients affect LRRK2, transfected various mutants into performed fractionation experiments. Unexpectedly, are collected both soluble fractions manner similar wild type (WT). I2020T mutant associates with rafts, WT. raft WT suggests that alteration on rafts contributes PD.