Differential Cell Line Susceptibility to the Emerging Novel Human Betacoronavirus 2c EMC/2012: Implications for Disease Pathogenesis and Clinical Manifestation

• DOI: 10.1093/infdis/jit123
• Publication Date: 2013-03-27T05:07:44Z
• Authors: Jasper Fuk-Woo Chan; Kwok-Hung Chan; Garnet Kwan-Yue Choi; Kelvin Kai-Wang To; Herman Tse; Jian-Piao Cai; Man Lung Yeung; Vincent Chi-Chung...; Honglin Chen; Xiao-Yan Che; Susanna Kar-Pui Lau; Patrick Chiu-Yat Woo; Kwok-Yung Yuen
• Keywords: Coronavirus; Viral Tropism; Virus Cultivation; Humans; Viral Load; Coronavirus Infections; Virus Replication; Cell Line; 3. Good health

The emerging novel human betacoronavirus 2c EMC/2012 (HCoV-EMC) was recently isolated from patients with severe pneumonia and renal failure associated an unexplained high crude fatality rate of 56%. We performed a cell line susceptibility study 28 lines. HCoV-EMC found to infect the respiratory tract (polarized airway epithelium Calu-3, embryonic fibroblast HFL, lung adenocarcinoma A549), kidney (embryonic HEK), intestinal (colorectal Caco-2), liver cells (hepatocellular carcinoma Huh-7), histiocytes (malignant histiocytoma His-1), as evident by detection or increasing viral load in culture supernatants, nucleoprotein expression immunostaining, and/or cytopathic effects. Although infected neuronal (NT2) monocyte T lymphocyte lines (THP-1, U937, H9) had increased loads, their relatively lower production corroborated absent This range tissue tropism is broader than that for all other HCoVs, including SARS coronavirus, HCoV-OC43, HCoV-HKU1, HCoV-229E, HCoV-NL63, which may explain mortality this disease. A recent showed can primate, porcine, bat therefore jump interspecies barriers. also civet rabbit These findings have important implications diagnosis, pathogenesis, transmission HCoV-EMC.