Simultaneous screening of zebrafish larvae cardiac and respiratory functions: a microfluidic multi-phenotypic approach

• DOI: 10.1093/intbio/zyac015
• Publication Date: 2022-11-23T11:01:26Z
• Authors: Arezoo Khalili; Ellen van Wijngaa...; Georg R Zoidl; Pouya Rezai
• Keywords: Cadmium chloride; Fish fin; Phenotypic screening; Ichthyoplankton

Abstract Multi-phenotypic screening of multiple zebrafish larvae plays an important role in enhancing the quality and speed biological assays. Many microfluidic platforms have been presented for phenotypic assays, but multi-organ larvae, from different needed orientations, a single device that can enable rapid large-sample testing is yet to be achieved. Here, we propose multi-phenotypic quadruple-fish chip simultaneous monitoring heart activity fin movement 5–7-day postfertilization trapped chip. In each experiment, movements four were quantified dorsal view terms beat frequency (FBF). Positioning optical prisms next traps provided lateral views enabled rate (HR) monitoring. The device’s functionality chemical was validated by assessing impacts ethanol on activities. Larvae treated with 3% displayed significant drop 13.2 35.8% HR FBF, respectively. Subsequent tests cadmium chloride highlighted novel application our effect heavy metals cardiac respiratory function at same time. Exposure 5 $\mu$g/l revealed increase 8.2% 39.2% employed monitor behavioral responses induced stimuli various applications such as ecotoxicology drug discovery.