High prevalence of -lactam and macrolide resistance genes in human oral Capnocytophaga species

• DOI: 10.1093/jac/dkt350
• Publication Date: 2013-09-08T00:35:40Z
• Authors: E. Ehrmann; T. Handal; Z. Tamanai-Shacoori; M. Bonnaure-Mallet; T. Fosse
• Keywords: Capnocytophaga

To determine macrolide–lincosamide–streptogramin (MLS) resistance determinants in the Capnocytophaga genus and to describe prevalence of β-lactam genes human oral species. Forty-eight isolates identified by analysis 16S rRNA sequences were isolated from subgingival samples 14 haematology patients (HPs), 11 periodontitis (PPs) 17 healthy volunteers (HVs). MICs MLS antibiotics obtained for all isolates. blaCfxA, blaCSP-1 (encoding a new class A β-lactamase) [erm(F), erm(B), erm(Q), erm(D), erm(C) erm(A)] evaluated using specific PCR sequencing. In HVs, which had lowest β-lactamase-producing comparison with other groups (16%; P < 0.001), ochracea was prominent species (68%; 0.03). PPs, high β-lactamase-positive (82%; sputigena more frequently (64%; HPs, 50% β-lactamase-positive. The rarely (15%) gingivalis, granulosa leadbetteri only PPs and/or HPs. All β-lactam-resistant (44%) PCR-positive blaCfxA (31%) or (12.5%). Interestingly, subgroup C. Twenty-nine percent resistant independently identification, β-lactamase production patient group. MLS-resistant carried erm(F) gene (93% 7%, respectively), previously unknown genus. Our findings illustrate that are important contributors reservoir microbiome.