LncRNA PCAT18/miR-301a/TP53INP1 axis is involved in gastric cancer cell viability, migration and invasion

• DOI: 10.1093/jb/mvaa079
• Publication Date: 2020-07-13T11:21:11Z
• Authors: Jin Dou; Daoyuan Tu; Haijian Zhao; Xiaoyu Zhang
• Keywords: Male; 0301 basic medicine; Mice, Inbred BALB C; Mice, Nude; Middle Aged; 3. Good health; Survival Rate; Mice; MicroRNAs; 03 medical and health sciences; Cell Movement; Stomach Neoplasms; Cell Line, Tumor; Disease Progression; Animals; Humans; Female; Genes, Tumor Suppressor; RNA, Long Noncoding; Carrier Proteins; Heat-Shock Proteins; Cell Proliferation; Signal Transduction

Abstract MiR-301a is as an oncogene involved in the regulation of gastric cancer (GC) progression, but the underlying mechanism is unclear. This study was to explore the lncRNA PCAT18/miR-301a/TP53INP1 axis in regulating the GC cell proliferation and metastasis. In the present study, GC tissues and cell lines were collected for the detection of PCAT18 expression. Herein, we found that PCAT18 is significantly decreases in human GC tissues and five GC cell lines. Overexpression of PCAT18 inhibits cell viability, invasion and migration of GC cells and tumour growth of GC xenograft tumours. PCAT18 negatively regulates the expression level of miR-301a. The interaction between PCAT18 and miR-301a is confirmed by RIP and RNA pull down. MiR-301a mimic increases cell viability and promotes cell migration and invasion and reverses the inhibitory action of PCAT18. TP53INP1 expression is negatively regulated by miR-301a and TP53INP1/miR-301a is involved in GC viability, migration and invasion. The promoting of PCAT18 on TP53INP1 expression is abolished by miR-301a overexpression. In conclusion, lncRNA PCAT18 acts as a tumour suppressor for GC and lncRNA PCAT18, miR-301a and TP53INP1 comprise a signal axis in regulating GC cell proliferation, migration and invasion.