Comparative expression profiling identifies differential roles for Myogenin and p38α MAPK signaling in myogenesis

• DOI: 10.1093/jmcb/mjs045
• Publication Date: 2012-07-31T06:44:18Z
• Authors: Qi-Cai Liu; Xiao-Hui Zha; Hervé Faralli; Hang Yin; Caroline Louis-Jeune; Eusebio Perdiguero; Erinija Pranckevi...; Pura Muñoz-Cànoves; Michael A. Rudnicki; Marjorie Brand; Carol Perez-Iratxeta; F. Jeffrey Dilworth
• Keywords: MEF2C; Cell fusion

Skeletal muscle differentiation is mediated by a complex gene expression program requiring both the muscle-specific transcription factor Myogenin (Myog) and p38α MAPK (p38α) signaling. However, relative contribution of Myog to formation mature myotubes remains unknown. Here, we have uncoupled activity from that gain insight into individual roles these proteins in myogenesis. Comparative profiling confirmed activates genes involved function. Furthermore, found absence signaling, leads down-regulation cell cycle progression. Consistent with this, sufficient induce exit. Interestingly, p38α-defective, Myog-expressing myoblasts fail form multinucleated myotubes, suggesting an important role for fusion. Through analysis up-regulated genes, tetraspanin CD53 was identified as candidate fusion protein, ex vivo primary myoblasts, during myofiber regeneration mice. Thus, our study has revealed unexpected mediating exit essential through up-regulation CD53.