Transcript profiling of Zea mays roots reveals gene responses to phosphate deficiency at the plant- and species-specific levels

• DOI: 10.1093/jxb/ern115
• Publication Date: 2008-05-25T00:13:30Z
• Authors: Carlos Calderon-V...; Enrique Ibarra-La...; Juan Caballero-Perez; Luis Herrera-Estr...
• Keywords: 0106 biological sciences; 2. Zero hunger; Transcription, Genetic; Nitrogen; Reverse Transcriptase Polymerase Chain Reaction; Gene Expression Profiling; Lipid Metabolism; Plant Roots; Zea mays; 01 natural sciences; Carbon; Phosphates; Phenotype; Species Specificity; Gene Expression Regulation, Plant; Oligonucleotide Array Sequence Analysis; Plant Proteins; Signal Transduction

Maize (Zea mays) is the most widely cultivated crop around world; however, it commonly affected by phosphate (Pi) deficiency in many regions, particularly acid and alkaline soils of developing countries. To cope with Pi deficiency, plants have evolved a large number developmental biochemical adaptations; for maize, underlying molecular basis these responses still unknown. In this work, transcriptional response maize roots to starvation at 1, 3, 6, 10 d after onset deprivation was assessed. The investigation revealed total 1179 Pi-responsive genes, which 820 363 genes were found be either up- or down-regulated, respectively, 2-fold more. involved various metabolic, signal transduction, gene networks. A set transcription factors, may potential targets breeding, identified. addition, expression profiles changes specific metabolites also correlated. results show that several dicotyledonous plant are conserved but some genetic appear more leads shift recycling internal roots. Ultimately, work provides comprehensive view Pi-responses model economically important cereals sets framework produce Pi-specific microarrays study global between Pi-efficient Pi-inefficient genotypes.